A couple of things I forgot above.
First, the links.
List of free tutorials
Open Helix tutorials
The UCSC Genome Browser introduction
The Genome Browser itself!
Next, after you enter TP53 in the "gene" box of the browser, you press the "submit" button. (There is no "jump" button here.)
Once you have zoomed in to see the actual base sequence, you will see what the mysterious lines below it are: the amino acid sequence. Each of the capital letters stands for an amino acid that would result if the three bases above were transcribed and then translated. Here is the genetic code, for convenience, and then a list of amino acid abbreviations. NOTE THAT YOU MUST READ IN THE DIRECTION OF THE ARROWS. In this stretch of DNA, that is right-to-left.
A alanine L leucine W tryptophan
B aspartate or asparagine M methionine (start) Y tyrosine
C cysteine N asparagine Z glutamine or glutamate
D aspartate P proline X any
E glutamate Q glutamine * translation stop
F phenylalanine R arginine - gap of indeterminate length
G glycine S serine
H histidine T threonine
I isoleucine U selenocysteine
K lysine V valine
A couple more things you may wonder about. EST stands for Expressed Sequence Tag, and is a short stretch of DNA that comes from from cDNA. cDNA is not DNA that was sequenced directly, but inferred by reverse-engineering a bit of mRNA (that is, writing out its DNA complement).
First, the links.
List of free tutorials
Open Helix tutorials
The UCSC Genome Browser introduction
The Genome Browser itself!
Next, after you enter TP53 in the "gene" box of the browser, you press the "submit" button. (There is no "jump" button here.)
Once you have zoomed in to see the actual base sequence, you will see what the mysterious lines below it are: the amino acid sequence. Each of the capital letters stands for an amino acid that would result if the three bases above were transcribed and then translated. Here is the genetic code, for convenience, and then a list of amino acid abbreviations. NOTE THAT YOU MUST READ IN THE DIRECTION OF THE ARROWS. In this stretch of DNA, that is right-to-left.
A alanine L leucine W tryptophan
B aspartate or asparagine M methionine (start) Y tyrosine
C cysteine N asparagine Z glutamine or glutamate
D aspartate P proline X any
E glutamate Q glutamine * translation stop
F phenylalanine R arginine - gap of indeterminate length
G glycine S serine
H histidine T threonine
I isoleucine U selenocysteine
K lysine V valine
A couple more things you may wonder about. EST stands for Expressed Sequence Tag, and is a short stretch of DNA that comes from from cDNA. cDNA is not DNA that was sequenced directly, but inferred by reverse-engineering a bit of mRNA (that is, writing out its DNA complement).
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